Propolis-Based Solution Application in Extraction Socket Healing

Saint-Joseph University

Status and phase: Completed; Phase 2; Phase 1

Conditions: Healthy

Treatments: Other: Saline rinse (control); Drug: Propolis-based topical solution (52% propolis extract, clove oil [Eugenia caryophyllus], and white willow bark [Salix alba] extract)

Study type: Interventional

Funder types: Other

Identifiers

NCT07665814

Tfemd-2026-54

Details and patient eligibility

About

This pilot study evaluated whether a propolis-based topical solution improves soft-tissue healing after tooth extraction. Thirteen patients requiring extraction of two comparable teeth on opposite sides of the mouth were enrolled in a split-mouth design, in which one extraction site received the propolis solution while the other served as an internal control. The test site was treated with the propolis solution three times daily for seven days, while the control site received only saline rinses. Healing was tracked using 3D intraoral scans obtained before extraction and on days 7 and 14 afterward, allowing the percentage of socket closure to be measured objectively over time. Postoperative pain was also assessed using a standard visual pain scale on days 1, 3, and 7. The study aimed to determine whether this propolis-based formulation could serve as a natural, low-cost adjunct to standard post-extraction care to support faster tissue repair.

Enrollment: 14 patients

Sex: All

Ages: 8+ years old

Volunteers: Accepts Healthy Volunteers

Inclusion criteria

• Cooperative adult patients willing to accept the study protocol
• Minors with a cooperative parent/legal guardian providing consent
• Non-surgical extraction of bilaterally positioned teeth
• Low-to-moderate extraction difficulty per the Pederson scale
• Systemically healthy, with no history of systemic disease

Exclusion criteria

• Non-consenting patients
• Failure to attend follow-up appointments per protocol
• Heavy smokers (>10 pack-years)
• Systemic disease (e.g., diabetes mellitus, immunosuppressive syndromes)
• Current use of contraceptive medication
• Systemic antibiotic therapy within the previous 3 months
• Anticoagulant therapy within the previous 4 weeks
• Requirement for antibiotic prophylaxis
• Pregnant or breastfeeding women
• Known allergy to honey or bee-related products
• Known allergy to local anesthetics used in dental procedures
• Higher-difficulty extractions requiring sutures or other hemostatic measures

Trial design

Primary purpose: Treatment

Allocation: Non-Randomized

Interventional model: Crossover Assignment

Masking: Single Blind

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14 participants in 2 patient groups

Extraction site treated with a propolis-based solution

Experimental group

Treatment:

Drug: Propolis-based topical solution (52% propolis extract, clove oil [Eugenia caryophyllus], and white willow bark [Salix alba] extract)

Contralateral extraction site treated with saline mouthwash only

Active Comparator group

Treatment:

Other: Saline rinse (control)