Total Lymphoid Irradiation Pre-HSCT in Severe Congenital Neutropenia

Federal Research Institute of Pediatric Hematology, Oncology and Immunology

Status and phase: Not yet enrolling; Phase 2

Conditions: GATA2 Deficiency; Severe Congenital Neutropenia

Treatments: Other: conditioning with TLI

Study type: Interventional

Funder types: Other

Identifiers

NCT04844177

NCPHOI-2021-02

Details and patient eligibility

About

Severe congenital neutropenia (SCN) is a group of primary immunodeficiencies caused by distinct gene mutations and characterized by neutrophil maturation impairment, which leads to neutropenia, predisposition to severe bacterial and fungal infections, and myeloid malignancies. Granulocyte-colony stimulation factor is used for pathogenetic therapy, however, no adequate response is seen in some patients.

The only curative option for SCN is hematopoietic stem cell transplantation (HSCT). An indication for HSCT in SCN is: no adequate response to G-CSF therapy, or development of malignancies, or found unfavorable mutations of SCN genes, leading to poor response to G-CSF and high risk of malignant transformation.

One of the major peculiarities of HSCT in SCN is a high risk of graft failure. That was described in few studies in SCN transplantation and was also observed in our SCN HSCT cohort. We also consider the role of TCRab/CD19 graft depletion, which is routinely used in our center for GVHD prophylaxis in increased risks of graft failure.

Another problem often observed in our patients is the relatively high risks of death of infections, developed after graft failure.

Due to predominantly early HSCT graft failure development, non-sufficient immuablation is presumed as the main reason for graft failure. Because of the low level of toxicity, associated with TCRab/CD19 depletion usage, this strategy is planned to be used in the current study. To increase an immunoablative potential of conditioning regimen in SCN, total lymphoid irradiation will be studied in combination with myeloablative agents and standardly used serotherapy.

Enrollment: 10 estimated patients

Sex: All

Ages: 18 months to 21 years old

Volunteers: No Healthy Volunteers

Inclusion criteria

• Clinical indications for HSCT in SCN: clinical diagnosis of SCN with (1) no adequate response to G-CST therapy or (2) with malignant transformation or (3) unfavorable mutations of known SCN genes
• GATA2 deficiency
• SCN patients age at HSCT 18 months - 21 years
• GATA2 deficiency patients age at HSCT more than 10 years
• Signed informed consent to participate in the study
• Presence of HLA-matched unrelated or HLA-mismatched related donor

Exclusion criteria

• Presence of HLA matched related donor in absence of pathologic SCN gene mutation
• Inability to perform TCRab/CD19 graft depletion
• Contraindications for HSCT due to patients somatic condition

Trial design

Primary purpose: Treatment

Allocation: N/A

Interventional model: Single Group Assignment

Masking: None (Open label)

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10 participants in 1 patient group

intervention/treatment

Experimental group

Description:

Total lymphoid irradiation 4 Gy (days -7, -6) in combination with: * Fludarabine 150 mg/m2 (days-6, -5, -4, -3, -2) * Cyclophosphamide 120 mg/kg (days -5, -4, -3) * Thymoglogulin (Genzyme) 5 mg/kg (days -5, -4) * Melphalan 180 mg/m2 (day -2) * Rituximab 100 mg/m2 (day -1) * Hematopoietic stem cell graft infusion after TCRab/CD19 depletion - day 0

Treatment:

Other: conditioning with TLI