Transcriptional and Epigenetic Program of PCOS Women (EPIC)

AdventHealth Translational Research Institute

Status

Active, not recruiting

Conditions

PCOS

Treatments

Other: MR assessment of Whole Body Fat

Procedure: Adipose tissue biopsy

Other: DEXA

Other: Magnetic Resonance (MR) Assessment of the Abdomen

Other: Oral glucose tolerance test

Study type

Observational

Funder types

Other

Identifiers

NCT04034706

1354588

Details and patient eligibility

About

The main goal is to reveal differences in global gene expression in two different adipose tissue (AT) depots of females with and without polycystic ovary syndrome (PCOS) and how these are influenced by upstream epigenetic markers

Enrollment

108 patients

Sex

Female

Ages

18 to 45 years old

Volunteers

Accepts Healthy Volunteers

Inclusion criteria

Women;
Age 18-45 years inclusive;
BMI 23-40 kg/m2 inclusive;
HbA1C ≤ 6.0% or fasting plasma glucose < 126 mg/dL;
Weight stable (± 3 kg) during the 3 months prior to enrollment;
Women must be > 9 months post-partum;
For PCOS: A documented history of PCOS from their physician according to the Androgen Excess (AE)-PCOS criteria: (defined by the presence of hyperandrogenism (clinical and/or biochemical), ovarian dysfunction (oligo-anovulation and/or polycystic ovaries), and the exclusion of related disorders (eg. hypoadrenalism, ovarian tumors)
Regular menstrual cycle for females without PCOS
Able to provide written, informed consent.

Exclusion criteria

Postmenopausal women
Women with hysterectomy
Pregnancy, lactation or < 9 months postpartum from the scheduled date of screening.
Fasting plasma glucose > 126 mg/dL, or HbA1c > 6% or diagnosis with Type 2 Diabetes (T2D) or Type 1 Diabetes (T1D)
History or presence of cardiovascular disease (unstable angina, myocardial infarction or coronary revascularization within 6 months, clinically significant abnormalities on EKG), presence of cardiac pacemaker, implanted cardiac defibrillator.
Liver disease (AST or ALT >2.5 times the upper limit of normal)
Kidney disease (creatinine >1.6 mg/dl or estimated GFR <60 ml/min)
Dyslipidemia, including triglycerides >500 mg/dl, LDL >200 mg/dl
Anemia (hemoglobin <11 g/dl)
Thyroid dysfunction (suppressed thyroid-stimulating hormone, elevated TSH <10 µIU/ml if symptomatic or elevated TSH >10 µIU/ml if asymptomatic)
Uncontrolled hypertension (BP >160 mmHg systolic or >100 mmHg diastolic)
Elevated hsCRP or known active infection
History of cancer within the last 5 years (skin cancers, with the exception of melanoma, may be acceptable)
History of drug or alcohol abuse (> 3 drinks per day) within the last 5 years. Current drug use may be determined by plasma or urine drug screens.
psychiatric disease prohibiting adherence to study protocol.
History of organ transplant.
Known history of HIV, active Hepatitis A, B or C or tuberculosis.
History of bariatric surgery.
Current smokers (smoking within the past 3 months).
Current use of beta-adrenergic blocking agents.
Use of oral or injectable anti-hyperglycemic agents: metformin, sulfonylureas, DPP IV inhibitors, SGLT-2 inhibitors, thiazolidinediones, acarbose, GLP-1 analogs and insulin unless willing to undertake a washout period of 15 days for metformin and GLP-1 analogs and undergo subsequent laboratory screening tests.
Gonadotropin Releasing Hormone (GnRH) and/or Antiandrogen use within the last 2 months.
Use of any medications known to influence fat and/or energy metabolism (eg growth hormone therapy, glucocorticoids [steroids], etc.)
Initiation or change in hormone replacement therapy within the past 3 months (including, but not limited to thyroid hormone, estrogen replacement therapy or In Vitro Fertilization therapy).
Current treatment with blood thinners or antiplatelet medications that cannot be safely stopped for biopsy and testing procedures.
Not able or willing to have DXA scans or are above the acceptable weight limit (450 lbs) of the DXA scanner.
Presence of any condition that, in the opinion of the Investigators, compromises participant safety or data integrity or the participant's ability to complete study visits.
Not able to participate in MRI assessments due to:
1. Metal implants (pacemaker, non-removable body piercings, aneurysm clips) based on Investigator's judgment at screening
2. Physical limitations or equipment tolerances (e.g., MRI bore size) based on Investigator's judgment at screening
3. Inability to tolerate MRI imaging without sedation or claustrophobia