Transcriptome Sequencing Analysis of Whole Blood From Patients With Trigeminal Neuralgia

Peking University

Status: Unknown

Conditions: Plasma; Blood; Trigeminal Neuralgia; Cerebrospinal Fluid

Study type: Observational

Funder types: Other

Identifiers

NCT04923399

2020PHB265-01

Details and patient eligibility

About

Trigeminal neuralgia is a type of neuropathic pain that brings great physical and psychological pressure to patients. Chronic pain can cause changes in the composition of central and peripheral body fluids, and these changes may be useful for the prediction and treatment of pain. In this study, the whole blood of patients with trigeminal neuralgia and non-chronic pain was collected, and transcriptome sequencing (RNA-seq) was performed to determine the peripheral transcriptome changes of trigeminal neuralgia (TN) patients. And compare the expression of neuropeptide Y (NPY) in plasma and cerebrospinal fluid of TN and non-chronic pain patients.

Full description

Trigeminal neuralgia is a kind of paroxysmal, knife-cutting, stubborn and severe pain in the facial distribution area of the trigeminal nerve caused by slight stimulation, which brings a great physical and psychological burden to the patient. Chronic pain can cause changes in the composition of central and peripheral body fluids, and these changes are very useful for the prediction and treatment of pain. In this study, the whole blood of patients with trigeminal neuralgia and non-chronic pain was collected for transcriptome sequencing (RNA-seq) sequencing. The results of transcriptome sequencing can derive the differentially expressed genes in the whole blood of TN patients and the pathways enriched by differentially expressed genes. At the same time, NPY (Neuropeptide y) plays an important role in head and face pain. We will also verify the difference in NPY expression in the cerebrospinal fluid and plasma of TN and non-chronic pain patients, and the correlation between NPY expression and pain.

Enrollment: 20 estimated patients

Sex: All

Ages: 20 to 75 years old

Volunteers: No Healthy Volunteers

Inclusion criteria

1. Patients with primary trigeminal neuralgia receive trigeminal nerve microvascular decompression, and healthy people without a history of chronic pain or patients with facial nerve palsy without chronic pain receive facial nerve decompression as a control;
2. Age from 30 to 60 years old
3. Complete white blood cell count: 4.8~11.0x109/L

Exclusion criteria

1. Patients with co-infection and tumor
2. Patients with craniocerebral disease

Trial design

20 participants in 2 patient groups

TN (Trigeminal Neuralgia) group

Description:

Trigeminal neuralgia patients undergoing trigeminal nerve microvascular decompression

Non-TN group

Description:

patients without chronic pain or history of chronic pain