Type VI Secretion System in Klebsiella Pneumoniae

Sohag University

Status: Enrolling

Conditions: Detection of Relation to Ab Resistance, Biofilm; Detection of Type VI Secretion System in K.Pneumoniae

Treatments: Diagnostic Test: Routine bacteriological cultures; Diagnostic Test: Molecular detection by PCR; Diagnostic Test: Identification of the organism and antibiotic sensitivity will be done by VITEK II; Diagnostic Test: Detection of biofilm formation

Study type: Observational

Funder types: Other

Identifiers

NCT05516654

Soh-Med-22-8-23

Details and patient eligibility

About

Klebsiella pneumoniae (K. pneumoniae) is an important opportunistic pathogen contributing to nosocomial and antimicrobial-resistant infections. The increasing prevalence of infections caused by multidrug-resistant K. pneumoniae has emerged as a major clinical and public health threat, while the serous organ and life-threatening infections caused by highly virulent K. pneumoniae have also emerged( Russo and Marr, 2019; Wyres et al., 2020).

Full description

Both drug-resistant and highly virulent K. pneumoniae have brought major challenges to clinical treatment and stimulated interest in studying K. pneumoniae. However, knowledge of the genomics, ecology, and pathogenicity of K. pneumoniae is relatively limited. Recently, the type VI secretion system (T6SS) was identified as a virulence factor in K. pneumoniae (Martin and Bachman, 2018). Furthermore, K. pneumoniae was found to exploit the T6SS nano-weapon to destroy bacterial competitors and fungi (Storey et al., 2020).

The bacterial Type VI Secretion System (T6SS) is a membrane-attached contractile phage tail that is physically and Mechanistically similar to a membrane-linked intracellular contractile phage tail. T6SS spike and tube elements, as well as anti-bacterial and anti-eukaryotic effectors, are propelled out of predatory T6SS positive cells and into target cells by a fast conformational shift in the structural framework of a sheath protein complex, according to recent research (Kudryashev et al., 2015).

However, the limited studies and lack of information on the T6SS in K. pneumoniae necessitate further exploration to clarify the physiological metabolism and pathogenic information of this clinically important bacterial species.

Enrollment: 70 estimated patients

Sex: All

Ages: 12 weeks to 90 years old

Volunteers: No Healthy Volunteers

Inclusion criteria

• All patients suffering from infections that can be caused by Klebsiella Pneumoniae.

Exclusion criteria

• Samples diagnosed to have organisms other than Klebsiella Pneumoniae