Understanding the Relationship Between Inflammation and Insulin Resistance in the Adipose Tissue

Mayo Clinic

Status: Completed

Conditions: Insulin Resistance; Obesity

Treatments: Procedure: Fat Aspiration; Dietary Supplement: Fish Oil Supplement; Procedure: Fat biopsy during scheduled abdominal surgery

Study type: Interventional

Funder types: Other

Identifiers

NCT02378077

14-001288

Details and patient eligibility

About

This study is being done to better understand the relationship between inflammation and insulin resistance in your Adipose (fat) Tissue .

Full description

This study is being done to determine whether eosinophils (a type of white blood cell) play an important role in human fat metabolism and inflammation.

This study is being done to determine if fish oil supplement could play a role in reducing inflammation and improving insulin sensitivity in your fat tissue.

Enrollment: 28 patients

Sex: All

Ages: 30 to 50 years old

Volunteers: Accepts Healthy Volunteers

Inclusion and exclusion criteria

Lean, healthy person with a body mass index (BMI) of 25 or less, or you are obese, BMI between 30 and 50, and non-diabetic.

Lean healthy controls will not be on any medication and will be undergoing elective abdominal surgery excluding those involving acute inflammation (i.e. acute cholecystitis, bowel perforation, or diverticulitis).

Obese, non-diabetic subjects will have a BMI between 30 and 50 and be taking no medications affecting glucose metabolism or lipid metabolism. These subjects will be identified from a pool of patients undergoing an elective abdominal surgical procedure such as inguinal hernia repair, laparoscopic Nissen fundoplication, planned cholecystectomy, but also bariatric surgery.

Volunteers will not be taking corticosteroid therapy or have a history of asthma, COPD or atopic syndrome. All subjects will undergo a medical history intake and a physical examination followed by an OGTT to confirm normal glucose.

Trial design

Primary purpose: Other

Allocation: Non-Randomized

Interventional model: Single Group Assignment

Masking: None (Open label)

---

28 participants in 2 patient groups

Lean or Obese, Non-Diabetic

Experimental group

Description:

To determine whether eosinophil content of adipose tissue is related to insulin sensitivity. We will use euglycemic clamps, fat biopsy (obtained during a scheduled abdominal surgery) and fat aspiration for analysis of subcutaneous (Sc) and omental (OM) adipose tissue from obese, insulin resistant and lean, insulin sensitive volunteers to test the hypothesis that, as in mice, eosinophil content in human subcutaneous and omental white adipose tissue, inversely correlates with body weight, with skeletal muscle and hepatic insulin sensitivity.

Treatment:

Procedure: Fat biopsy during scheduled abdominal surgery

Procedure: Fat Aspiration

Fish oil supplementation

Experimental group

Description:

Determine whether, in adipose tissue, levels of, anti-inflammatory molecules correlate with insulin sensitivity and whether these levels are altered by a treatment designed to promote resolution of inflammation. Volunteers will take a fish oil supplement for three months.

Treatment:

Dietary Supplement: Fish Oil Supplement

Procedure: Fat Aspiration