Urinary Actin, as a Potential Marker of Sepsis-related Acute Kidney Injury

University of Pecs

Status: Completed

Conditions: Acute Kidney Injury Due to Sepsis; Sepsis

Treatments: Other: Sepsis therapy; Other: Sepsis-related acute kidney injury therapy

Study type: Observational

Funder types: Other

Identifiers

NCT04968262

no. 4327.316-2900/KK15/2011

KA-2018-17 (Other Grant/Funding Number)

Details and patient eligibility

About

In our study, 17 septic, 43 sepsis-related acute kidney injury and 24 control patients were enrolled. Blood and urine samples were collected at the intensive care unit from acutely diagnosed septic and sepsis-related acute kidney injury patients at three time points (T1-3): T1: within 24 hours after admission; T2: second day morning; T3: third day morning of follow-up. Patients with malignancies needing palliative care, end-stage renal disease or kidney transplantation were excluded. Not more than one sample (venous blood, midstream spot urine) was collected from control patients. Serum and urinary actin levels were determined by quantitative Western blot. Urinary actin concentrations were expressed as µg/L, while serum actin levels were expressed as mg/L. Data were compared with laboratory and clinical parameters. Patients were categorized by the Sepsis-3 definitions and 30-day mortality data were investigated.

Full description

Actin is a globular protein present in every cell with a 42 kilodalton molecular mass. It plays an important role in muscle contraction while being an essential component of the cytoskeleton as well. Several proteins (e.g. gelsolin, Gc-globulin) bind actin in the circulation during the physiological cell turnover, thus making its urinary appearance unlikely. However, recent studies indicate that actin could be detected in the urine of kidney-transplant patients with acute kidney injury. Therefore, the main focus of our research was the detection and measurement of actin in the blood and urine in patients with sepsis or sepsis-related acute kidney injury, as the early recognition of kidney injury - especially in sepsis - is essential in the aspect of therapy and survival.

Enrollment: 84 patients

Sex: All

Ages: 18 to 90 years old

Volunteers: No Healthy Volunteers

Inclusion criteria

• Sepsis
• Sepsis-related acute kidney injury

Exclusion criteria

• malignancies needing palliative care
• end-stage renal disease
• kidney transplantation
• under 18 years of age
• unobtainable consent

Trial design

84 participants in 2 patient groups

Sepsis

Description:

Patients receive sepsis therapy.

Treatment:

Other: Sepsis therapy

Sepsis-related acute kidney injury

Description:

Patients receive sepsis and sepsis-related acute kidney injury therapy.

Treatment:

Other: Sepsis-related acute kidney injury therapy