Use of Microscopy, Cultures and Molecular Biological Methods for Diagnosing Nosocomial Pneumonia

Zealand University Hospital

Status: Completed

Conditions: Nosocomial Pneumonia

Treatments: Diagnostic Test: Microbiological diagnostics on pulmonary secretion

Study type: Observational

Funder types: Other

Identifiers

NCT04279873

REG-48-2017 (Other Identifier)

REG-048-2014

Details and patient eligibility

About

Nosocomial pneumonia in the Intensive Care Unit is often not properly diagnosed mainly due to ongoing antimicrobial therapy. The study investigates the feasibility of more advanced diagnostic technics.

Full description

ICU patients fulfilling the CDC criteria for nosocomial pneumonia and mechanically ventilated for no more than 24 hours are included in the study. The material for the diagnostic procedure is pulmonal secretion collected by 1. suctioning in the tracheal tube and 2. bronchioalveolar lavage. The diagnostic procedures are culturing, 16S and18S gene PCR and subsequent sequencing. The 3 diagnostic methods will be compared in order of proper diagnosis and the ability to determine a specific antimicrobial treatment.

Enrollment: 22 patients

Sex: All

Ages: 18+ years old

Volunteers: No Healthy Volunteers

Inclusion criteria

• Patients intubated because of respiratory failure following nosocomial pneumonia

Exclusion criteria

• Terminal patients
• Patients with lung cancer
• Intubated for more than 24 hours at the sampling time
• Not fulfilling the CDC criteria for nosocomial pneumonia

Trial design

22 participants in 1 patient group

Adults with nosocomial pneumonia

Description:

Diagnostic procedures on pulmonary secretion collected by tracheal suctioning and bronchoalveolar lavage.

Treatment:

Diagnostic Test: Microbiological diagnostics on pulmonary secretion