Use of Nitric Oxide Metabolites for Predicting Embryo Quality in In-Vitro Fertilization (IVF)

Johns Hopkins University

Status: Terminated

Conditions: Infertility

Treatments: Procedure: Day 5 transfer; Procedure: Transfer of embryos on day 3 or day 5 after fertilization.

Study type: Interventional

Funder types: Other

Identifiers

NCT00788619

NA_00013752

Details and patient eligibility

About

Improvements in In-Vitro Fertilization (IVF) outcomes have largely been due to the generation of excess embryos allowing for multiple embryo transfer. Unfortunately this has also increased the risk of multiple pregnancy with associated maternal and neonatal morbidity and mortality. The investigators believe that the concentration of nitric oxide metabolites measured in the culture medium from the first day of culture can identify the best quality embryos allowing us to transfer a limited number of embryos without compromising the optimum chance for pregnancy.

Enrollment: 2 patients

Sex: Female

Ages: 21 to 37 years old

Volunteers: Accepts Healthy Volunteers

Inclusion criteria

• Age 21-37
• Female
• Less than two previous unsuccessful IVF cycles

Exclusion criteria

• Day 3 FSH >9.9
• Fewer than 8 mature oocytes retrieved
• Fewer than 8 normally fertilized embryos on day 2 after fertilization

Trial design

Primary purpose: Treatment

Allocation: Randomized

Interventional model: Parallel Assignment

Masking: None (Open label)

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2 participants in 2 patient groups

Day 3 transfer

Active Comparator group

Description:

Subjects in this arm will have two embryos transferred three days after fertilization. Embryos will be selected based on the concentration of nitric oxide metabolites in the culture medium.

Treatment:

Procedure: Transfer of embryos on day 3 or day 5 after fertilization.

Day 5 transfer

Active Comparator group

Description:

Subjects will have two embryos transferred on day 5 after fertilization with selection of embryos based on morphologic criteria.

Treatment:

Procedure: Day 5 transfer