Utilizing Free DNA in Embryo Culture for PGT

Al Baraka Fertility Hospital

Status: Completed

Conditions: Genetic Diseases, Inborn; Mutation; Chromosome Abnormalities

Treatments: Procedure: NGS reading for Media Free DNA

Study type: Observational

Funder types: Other

Identifiers

NCT02884063

NGS-PGT

Details and patient eligibility

About

In the way for developing and optimizing protocol to be used as non- invasive methodology used as routine testing for PGS.

This protocol is to be adapted to replace the using of life embryo cells for genetic testing and aneuploidy study as well as for any type of genetic testing including single gene disorder or HLA typing or study.

Full description

Compare 100 embryos studied using NGS for chromosomal aneuploidies, and compare the results came from free media DNA with this results.

Enrollment: 100 patients

Sex: All

Ages: 20 to 45 years old

Volunteers: No Healthy Volunteers

Inclusion criteria

• all abnormal/aneuploidy embryos rejected for embryo transfer.

Exclusion criteria

• all embryos had unsuccessful diagnosis with blastomere biopsy.

Trial design

100 participants in 2 patient groups

NGS reading for Media Free DNA

Description:

Ability to read the DNA product available in embryo culture media using NGS to have valuable diagnostic image for genetic composition of embryo before implantation.

Treatment:

Procedure: NGS reading for Media Free DNA

NGS reading for blastomer DNA

Description:

DNA extracted from Blastomere used for aneuploidy screening.

Treatment:

Procedure: NGS reading for Media Free DNA