Vascular Endothelial Growth Factor Levels in Ectopic Pregnancy (VEGF)

Bagcilar Training and Research Hospital

Status: Completed

Conditions: Complication Following Ectopic Pregnancy

Study type: Observational

Funder types: Other

Identifiers

NCT01601964

BEH GynObs-1

Details and patient eligibility

About

The aim of this study is to determine plasma Vascular Endothelial Growth Factor level and change in patient with ectopic pregnancy who needs to surgical treatment.

Patient with ectopic pregnancy diagnosed and treated with medically or surgically will be included in the study. This is an observational study.The plasma Vascular Endothelial Growth Factor level will be measured first day and two day after the diagnosis of ectopic pregnancy.

Full description

This study is a prospective, observational, single-center study. Ethical approval was obtained from the Institutional Review Board. This study will be completed with 30 patients with ectopic pregnancy. An intervenous cannula will be centrifuged for 15 minutes at 1000 g within 30 minutes of collection. Aliquots of plasma will be added to eppendorf tubes and stored at -20°C.

Plasma Vascular Endothelial Growth Factor levels will be measured using a Human VEGF-A Platinum ELISA kit (bioscience company, Catalog No: 00122, Santa Clara, California, USA). All assays will be performed according to the manufacturer's instructions. The test kit sensitivity is 2.5 pg/ml. Intracycle variability is %4-6, intercycle variability is %8-10.

Enrollment: 30 patients

Sex: Female

Ages: 18 to 46 years old

Volunteers: No Healthy Volunteers

Inclusion criteria

• Patients who suspected ectopic pregnancy

Exclusion criteria

• hemodynamically unstable patient
• healthy pregnancy (a healthy increase in ß HCG)
• missed abortion
• incomplete abortion
• The patient who need immediate surgical treatment

Trial design

30 participants in 2 patient groups

Follow-up or medical treatment.

Description:

medical treatment

Surgical treatment

Description:

Surgical treatment