Vegetables Intake and Polymorphism TAS2R38 Gene by Healthy Adults

Poznan University of Life Sciences

Status: Completed

Conditions: Healthy Lifestyle; Diet Habit; Taste Disorders

Treatments: Behavioral: Control Group 1 personalized nutritional recommendations; Behavioral: Intervention genotype personalized nutritional recommendations; Behavioral: Control Group 2 general nutritional recommendations

Study type: Interventional

Funder types: Other

Identifiers

NCT04145453

UKB 872/19

Details and patient eligibility

About

Personalized nutrition is one of the most up to date trends in human nutrition and gains much interest of general public and scientists as well. Although we have gained some knowledge on gene-trait associations, the real effectiveness and usefulness of genotype-based nutritional recommendations is unknown. Many personalized nutrition companies are on the market today, some of them use personalized nutrition based on genotype analysis. For this reason, scientific basis of this approach should be clarified.

Our project can thus increase knowledge which can be applied in dietary counseling practice. Although we focus on increase vegetable and fruits intake, the study is designed as a proof of concept.

Full description

In humans, the TAS2R38 receptor gene is responsible for differences in the perception of bitter taste. This gene codes for a G protein that is associated with a flavor receptor regulated by phenylthiocarbamide (PTC) and propylthiouracil (PROP) ligands, which by binding to the receptor determines the degree of bitter taste. Cruciferous vegetables contain glucosinolates and isothiocyanates, which resemble PTC and PROP and thereby affect their perception of bitter taste through the TAS2R38 regulated receptor.

The polymorphism of this gene allows to distinguish three phenotypes:

• insensitive to bitter taste [bitter-non tasters]
• moderately sensitive to bitter taste [intermediate-bitter tasters]
• sensitive to bitter taste [bitter taster] Previous studies have shown that people who are carriers of one PAV haplotype experience a bitter taste more than AVI / AVI homozygotes, which are less sensitive to bitter taste. Hence, the TAS2R38 gene polymorphism is associated with nutritional decisions, including choice of vegetables and coffee.

Aim of the study is to verify effectiveness of the genotype based dietary intervention in people with or without polymorphism of TAS2R38 gene.

Enrollment: 174 patients

Sex: All

Ages: 18 to 60 years old

Volunteers: Accepts Healthy Volunteers

Inclusion criteria

• healthy

Exclusion criteria

• injuries, chronic diseases (e.g. diabetes, metabolic syndrome, cancer, hyperthyroidism), recent diet, pregnancy, breastfeeding, limited communication to the extent that no nutritional history can be carried out, eating disorders (according to nutritional history)

Trial design

Primary purpose: Basic Science

Allocation: Randomized

Interventional model: Parallel Assignment

Masking: Single Blind

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174 participants in 3 patient groups

intervention group

Experimental group

Description:

Participants with PAV haplotype will receive personalised dietary recommendations regarding consumption of fruit and vegetables. The information about genotype will be given at the beginning of study.

Treatment:

Behavioral: Intervention genotype personalized nutritional recommendations

control group 1

Sham Comparator group

Description:

Participants with PAV haplotype will receive personalised dietary recommendations regarding consumption of fruit and vegetables, but the information about genotype will be given at the end of study.

Treatment:

Behavioral: Control Group 1 personalized nutritional recommendations

Control group 2

Active Comparator group

Description:

Participants with AVI haplotype will receive general recommendations regarding consumption of fruit and vegetables.

Treatment:

Behavioral: Control Group 2 general nutritional recommendations