Viral and Microbial Circulation Between Humans, Domesticated and Wild Animals Along an Ecotone, Democratic Republic of Congo (MICROTONE)

Pasteur Institute

Status: Completed

Conditions: Zoonotic Disease

Treatments: Biological: stool collection; Biological: blood sampling; Other: Participatory activity and contact investigation; Other: Anthropological and historical interviews

Study type: Interventional

Funder types: Other; Industry

Identifiers

NCT04012164

2017-085

Details and patient eligibility

About

This study will evaluate the overlap between the intestinal microbiome and virome of wild and domesticated animals and human beings living in close proximity in three sites along an ecotone (ecological gradient) in the Democratic Republic of Congo.

Full description

The MICROTONE study sheds light on zoonotic disease emergence by examining social and ecological pathways facilitating microbial and viral flows between people and selected wild and domesticated animals along a gradient of ecological change in a forest-savanna mosaic in the Democratic Republic of Congo, an epicenter of zoonotic disease emergence. The investigators analyze potential viral and bacterial overlap among humans and animals and explain this overlap (or not) through social sciences and ecological analyses of human and animal mobilities, practices and contacts. This multi-disciplinary, multi-species investigation in an ecotone (a transitional ecological zone linked to zoonotic disease emergence) offers a "pre-history" of spillover and emergence, tracing an ecological zeb of virome and microbial sharing among humans and animals. It will elucidate why such microbial and viral flows occur.

To conduct this investigation,there are two human sub-studies: the social sciences participatory study; and the clinical study. The clinical study will involve 30 human subjects from whom blood and stool samples will be collected. The social sciences participatory study will involve self-collected activity and animal contact data and oral interviews among 60 human subjects.

Enrollment: 60 patients

Sex: All

Ages: 18+ years old

Volunteers: Accepts Healthy Volunteers

Inclusion criteria

• Age 18 years or older
• Accept participation in study
• Residing in one of three villages selected
• In good health and able to conduct habitual daily activities
• Accept self-collection of 2g of stool
• Accept blood collection (2-2.5 ml) by a medical professional
• Accept to self-collect daily activities and contacts with selected wild and domesticated animals for five months
• Accept to participate in an anthropological-historical interview on changing practices and contacts with wild and domesticated animals

Exclusion criteria

• Vulnerable adults will not be included
• Women who are pregnant or nursing at the time of recruitment and inclusion will not be included.
• Adults declaring themselves ill and unable to conduct their habitual daily activities will not be included.
• Adults with a chronic illness will not be included
• Adults who have a family member already included in the study will not be included.
• Adults refusing either recording of interviews or note-taking during interviews will not be included.

Trial design

Primary purpose: Basic Science

Allocation: N/A

Interventional model: Single Group Assignment

Masking: None (Open label)

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60 participants in 1 patient group

Microbiological, anthropological and historical study

Experimental group

Description:

30 adult participants will be recruited to self-report daily activities and contacts with domesticated and wild animals for a five month period. Following the five months of data collection, we will collect 5ml blood and 2g stool from each participant. From the fifth month of investigation, an additional 30 adult participants will participate in oral anthropological, historical interviews to develop the socio-historical context of their changing activities and relations with selected domesticated and wild animals. No other intervention will be performed.

Treatment:

Other: Participatory activity and contact investigation

Biological: blood sampling

Biological: stool collection

Other: Anthropological and historical interviews