Visualize Nociceptor Changes in Neuropathic Human

National Taiwan University

Status: Unknown

Conditions: Peripheral Neuropathy; Chemotherapy-induced Peripheral Neuropathy

Treatments: Diagnostic Test: harmonic generation (HGM) microscopic methods.

Study type: Interventional

Funder types: Other

Identifiers

NCT03112057

201610037DINC

Details and patient eligibility

About

The purpose of this experiment is to establish a solid medical foundation for the successful development of non-invasive harmonic generation (HGM) microscopic slicing methods. The purpose of this new technology platform is to directly observe the nerve endings of nociceptors To enhance understanding of how neuropathies occur, persistence, and ease. This technique will assist in the diagnosis and prognosis of patients with neuralgia.

Full description

This study will be the skin tissue for the virtual slice object, the study intends to the following research:

The number of people enrolled in the Court is 170.

Between 20 and 90 years old, meet the following conditions, suitable for participation in this test:

1. Healthy subjects of different ages (20 persons): to study the optical imaging of the skin and nerve endings: the control of healthy subjects and peripheral neuropathy patients skin observed by the optical images of the shoots.
2. Abandoned extra digit specimen of polydactyly(10 persons): to study the optical imaging of the normal skin and nerve endings in extra digit : collect abandoned digit specimen after surgical removal in polydactyly.
3. has been diagnosed with peripheral neuropathy (90 persons):to study the diagnosis of peripheral neuropathy of the skin nerve endings of the optical image observation and quantitative sensory nerve examination
4. clinically must be diagnosed by the skin slices of diabetic neuropathy patients have been removed after the completion of clinical diagnosis of the specimen,And then the skin slices of the nerve endings of the optical image observation (20 persons):study the technical observation of diabetic patients with skin sections of the nerve endings of the optical image observation: the clinical must be skin slices for the diagnosis of diabetic neuropathy, patients has been taken out after the completion of clinical diagnosis, and then the skin slices of the nerve endings of the light learning image observation.
5. because of chemotherapy during chemotherapy, is expected to have peripheral neuropathy: before the occurrence of peripheral neuropathy , Peripheral nerve lesions were cured after subcutaneous nerve optical observation (30 persons):for different periods of peripheral neuropathy subcutaneous nerve optical observation: in the peripheral neuropathy occurred before the occurrence of peripheral neuropathic symptoms, peripheral neuropathy after cure for subcutaneous nerve optical observation.

This test can not be attended if:

Those who suffer from systemic infection or have been infected with the site by the physician assessment is not suitable for examination.

Enrollment: 170 estimated patients

Sex: All

Ages: 20 to 90 years old

Volunteers: Accepts Healthy Volunteers

Inclusion criteria

• Healthy subjects of different ages
• abandoned extra digit specimen in polydactyly
• diagnosed with peripheral neuropathy
• diabetic neuropathy patients have been removed after the completion of clinical diagnosis of the specimen, And then the skin slices of the nerve endings of the optical image observation
• because of chemotherapy during chemotherapy, is expected to have peripheral neuropathy: before the occurrence of peripheral neuropathy , after cured peripheral nerve lesions

Exclusion criteria

• systemic infection or have been infected with the site by the physician assessment is not suitable for examination.

Trial design

Primary purpose: Diagnostic

Allocation: Non-Randomized

Interventional model: Parallel Assignment

Masking: None (Open label)

---

170 participants in 5 patient groups

Healthy subjects

Experimental group

Description:

Healthy subjects of different ages (20 persons), Interventions: harmonic generation microscopy

Treatment:

Diagnostic Test: harmonic generation (HGM) microscopic methods.

peripheral neuropathy

Experimental group

Description:

the participant have symptoms and diagnosed with peripheral neuropathy (90 persons), Interventions:harmonic generation microscopy

Treatment:

Diagnostic Test: harmonic generation (HGM) microscopic methods.

diabetic neuropathy

Experimental group

Description:

the participant have symptoms and diagnosed with diabetic neuropathy (20 persons), Interventions:harmonic generation microscopy

Treatment:

Diagnostic Test: harmonic generation (HGM) microscopic methods.

chemotherapy induced neuropathy

Experimental group

Description:

before chemotherapy, during chemotherapy, after peripheral nerve lesions were cured (30 persons). Interventions:harmonic generation microscopy

Treatment:

Diagnostic Test: harmonic generation (HGM) microscopic methods.

Polydactyly

Experimental group

Description:

Abandoned extra digit specimen of polydactyly(10 persons): the normal skin and nerve endings in extra digit: Interventions: harmonic generation microscopy

Treatment:

Diagnostic Test: harmonic generation (HGM) microscopic methods.