Yeast Cells as Antioxidant-Producing Probiotics

Benha University

Status and phase: Completed; Phase 1

Conditions: Infertility, Male

Treatments: Dietary Supplement: Vit C 1; Dietary Supplement: Vit C 3; Dietary Supplement: Vit C 2; Dietary Supplement: Yeast; Dietary Supplement: Vit E

Study type: Interventional

Funder types: Other

Identifiers

NCT02814695

HAWAA-001

Details and patient eligibility

About

The purpose of this study is to evaluate the efficacy of Baker's yeast extractions , probiotic antioxidant, in scavenging the oxidative stress status mediated damage on sperm motility,progressive motility and vitality as well as comparing this potency to that of Vitamin E in infertile men.

Full description

The practical part of the study took place from August 2020 to November 2021. The Study population was made up of infertile males attending Hawaa fertility center, banha city, Qaliubiya, Egypt.

40 study samples were collected from patients who indicated willingness to participate in the study and have had 3 to 7 days of sexual abstinence, using the masturbation method and ejaculated in wide mouthed plastic container as described by World Health Organization (WHO).

Samples were allowed to liquefy for 20 minutes and were examined for volume, viscosity, spermatozoa count by Neubauer haemocytometer , oxidative stress level measurement by Oxisperm, percentage of progressive motility and non-progressive motility and sperm vitality.

The Semen specimens were divided into ten equal fractions. First fraction was control, 0.1ml of liquefied semen mixed with 0.1ml Ham's F10 medium and incubated at 37o C for 30 minutes., 2nd: Vit. E, 3rd (Yeast extraction ( YE).Vitamin C was obtained from (Sigma, Germany), 3rd, 4th, 5th fractions (Vit. C. 1,2,3), 0.1ml liquefied semen was mixed with 0.1ml Ham's F10 medium supplemented with (0.02, 0.04, 0.06 mg/ml) Vitamin C respectively and incubated at 37o C for 30 minutes.

After semen specimen treated in all fractions, they were examined and assessed for microscopical changes, total motility, progressive motility, vitality, level of oxidative stress (OS) were detected in all fractions 30 minutes later after addition of the antioxidants.

Enrollment: 40 patients

Sex: Male

Ages: 22 to 35 years old

Volunteers: No Healthy Volunteers

Inclusion criteria

• must have been married for 1 year before inclusion into the study and were unable to achieve pregnancy
• must have not received an antibiotic treatment for the last 4 weeks prior to sampling
• showed oxidative stress- mediated asthenozoospermia in their semen

Exclusion criteria

• Severe male Factor

Trial design

Primary purpose: Basic Science

Allocation: Non-Randomized

Interventional model: Parallel Assignment

Masking: Double Blind

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40 participants in 4 patient groups

Control

No Intervention group

Description:

First fraction was control, 0.1ml of liquefied semen mixed with 0.1ml Ham's F10 medium and incubated at 37o C for 30 minutes.

Vit E supplementation

Experimental group

Description:

0.1ml liquefied semen was mixed with 0.1ml Ham's F10 medium supplemented with (2 mg/ml) Vitamin E and incubated at 37o C for 30 minutes.

Treatment:

Dietary Supplement: Vit E

Yeast supplementation

Experimental group

Description:

0.1ml liquefied semen was mixed with 0.1ml Ham's F10 medium supplemented with (20 mg/ml) Yeast extraction and incubated at 37o C for 30 minutes.

Treatment:

Dietary Supplement: Yeast

Vit C supplementation

Experimental group

Description:

fractions (Vit. C. 1,2,3), 0.1ml liquefied semen was mixed with 0.1ml Ham's F10 medium supplemented with (0.02, 0.04, 0.06 mg/ml) Vitamin C respectively and incubated at 37o C for 30 minutes.

Treatment:

Dietary Supplement: Vit C 2

Dietary Supplement: Vit C 3

Dietary Supplement: Vit C 1