Zinc and the Synthesis of Zinc Binding Proteins (Protocol B)

University of Florida

Status

Completed

Conditions

Zinc Deficiency

Treatments

Other: Acclimation

Other: Dietary zinc depletion

Other: Dietary zinc repletion

Study type

Observational

Funder types

Other

NIH

Identifiers

NCT01221129

R01DK031127 (U.S. NIH Grant/Contract)

UF-GCRC-772

Details and patient eligibility

About

The specific aim of this protocol is to examine differential expression of specific mRNAs and proteins that occur when the dietary intake of zinc is acutely reduced below the dietary requirement for a period of ten days.

Full description

Methods to assess the dietary zinc status of individual humans or populations are lacking. The study is a three period observational study of biomarkers of zinc status before, during and after zinc depletion. The entire dietary study will take 24 days composed of three parts:

Dietary Acclimation (Phase 1): 7 days
Dietary Zinc Restriction (Phase 2): 10 days
Dietary Zinc Repletion (Phase 3): 7 days

The dietary treatments and all blood draws will be carried out in the General Clinical Research Center (GCRC) at University of Florida. Previous research from has shown that both metallothionein gene expression and specific zinc transporter genes in blood leukocyte subsets are directly proportional to dietary zinc intake. By using cDNA array analysis and quantitative PCR technology with blood RNA, those genes most sensitive to zinc nutrition will be identified for the purpose of developing zinc status assessment methods on how zinc functions in human biology.

Additionally, buccal mucosal cells will be collected in the GCRC by using swabs, i.e., in a noninvasive manner, to further characterize the expression pattern of the genes under study. This method has the potential for nutrition studies in the field (international nutrition, etc).

Enrollment

10 patients

Sex

Male

Ages

21 to 35 years old

Volunteers

Accepts Healthy Volunteers

Inclusion criteria